Abstract

While melting analysis of short DNA amplicons are nothing new, some key improvements to the technique has
opened up some additional niche applications for which it can be applied. 

These aspects are :
------------------------
1) new fluorescent dyes

2) data capture rate and

3) analysis software for normalisation between samples. 

Software for High Resolution Melting (HRM) on the 7500 Fast Real time PCR system is now available, enabling
more sophisticated melting analysis with an easy to follow workflow and minimal subjective data analysis steps.

This presentation will briefly cover how HRM works, what it is being applied to and how it can fit into your
genomics projects. 

Some of the key features of the Applied Biosystems HRM Software, such as its auto calling capacity to minimizes subjective analysis, customizable multi-plot views, expandable windows and one–click colour assignment and how
we remove the requirement of a temperature shift to distinguish between homozygous mutants and wild type
more easily will be covered.

Register Now