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INDEX:
SDS news bulletin: AbD service upgraded--TaqPipe
v1.1
SDS Bulletin: 7000 SDS software V1.0.1 release
SDS News Bulletin- AB US$250K SNP Grant Announced
SDS Bulletin: cDNA Archive Kit
SDS Bulletin-IMPORTANT-TaqMan Probes/Primers Now
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SDS
news bulletin: AbD service upgraded--TaqPipe v1.1
SDS Bulletin
March 24, 2003
____________________________________________________________
Applied Biosystems has upgraded the Assays-by-Design
Service
____________________________________________________________
Applied Biosystems has today upgraded the TaqMan
SNP and Gene Expression design software used by the Assays-by-Design
(AbD) service. The new version (1.1) incorporates significant improvements
evolved and developed during the design, testing and manufacture
of over of 200,000 Assays-on-Demand products.
Specifically, Tm and quality parameters have been
improved. These enhancements to the design software can improve
a less efficient assay in some cases and, in other cases, fail assays
at the design stage before they reach manufacturing. Failing these
borderline assays further guards against delivering any poorly performing
assays.
If you wish to REORDER a SNP or Gene Expression
assay it is important to follow the procedure below. Do not reorder
an AbD product by resubmission of the target DNA sequence unless
you wish to have the assays redesigned. In most cases, the new version
of design software will create an assay with different primer and
probe sequences.
To receive an assay identical to the assay from
a previous order, you will need to construct an e-mail containing
the following four pieces of information:
1. The assay ID for each assay you are reordering.
This information can be found on your current assay tube, or in
the electronic data sheet on the CD-ROM you received with your original
order.
2. The sales order number(s) from your original
order, which can be found on the plate, packing slip, CD-ROM, and
the invoice.
3. The part number for the specific assay type you
are reordering.
4. Account information such as contact, purchase
order, credit card, bill-to and ship-to information Assays-by-Design
(AbD) service frequently asked questions:
__________________________________________________________
Q: What is the AbD service?
A: AbD lets you tap into Applied Biosystems' advanced
in-house TaqMan assay design and production pipeline. All you need
to do is submit DNA sequence information and Applied Biosystems
will design, synthesis, formulate and deliver a superior TaqMan
MGB assay. Either single-probe AbD assays (e.g. for gene expression)
and dual-probe AbD assays (e.g. For SNP analysis) can be synthesised
in small medium or large scales. Assays are delivered in the now
familiar single tube format in which the primers and probe)s) are
premixed together (just as they are with all the Assays-on-Demand
human and mouse products).
Q: Are AbD designs better than Primer Express designs?
A: Yes, because AbD uses more advanced design algorithms
than the current Primer Express.
Q: How do I optimise and AbD assay?
A: You don't have to. All assays are designed and
formulated to work without optimisation in TaqMan universal Master
Mix run under Universal Thermal Cycling Conditions.
Q: How do I order AbD assays?
A: Ordering AbD assays is simple. Use the freeware
program File Builder V2.0 (or higher) to create and check your sequence
submission files. Once you're ready, you can order assays by uploading
the sequence files directly to the Applied Biosystems store from
within File Builder.
Q: Where do I get File Builder?
A: It can be downloaded from: http://www.appliedbiosystems.com/support/software/assaysbydesign/installs.cfm
File Builder versions are available for Windows,
Mac OS X and Mac OS 9.0
Q: How much does AbD cost?
A: A small scale single-probe AbD assay (e.g. For
gene expression) costs about the same as a small scale custom TaqMan
probe. A dual-probe assay (e.g. For SNP detection) costs only about
20% more. For current prices please request a new SDS price list.
AbD: go to http://www.appliedbiosystems.com
then search for "Assays-on-Demand"
AoD GX: http://www.allgenes.com
AoD SNPs: http://www.allsnps.com
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SDS
Bulletin: 7000 SDS software V1.0.1 release
SDS Bulletin
March 20, 2003
_______________________________________________________
ABI PRISM. 7000 Sequence Detection System version
1.0.1
_______________________________________________________
Please note that a new version of 7000 system software
SDS v1.0.1 is now available for download on our website.
Software Release Notes
IMPORTANT:
SDS version 1.0.1 software resolves the following issue:
In version 1.0, the software fails to print all
lines of data in the printed report of an Absolute Quantitation
assay whenever the entire plate is selected. This version corrects
the printing software bug.
When upgrading to version 1.0.1, ensure that your
current software is version 1.0 (You can check this in the "About
ABI PRISM® 7000 SDS Software" under the Help menu).
To install the version 1.0.1, simply log into your
instrument PC as Administrator. Unzip the downloaded compressed
file. Double click on the setup executable. The installation program
will initiate; follow the simple instructions presented.
To download SDS v1.0.1, go to: http://www.appliedbiosystems.com/support/software/7000/updates.cfm
This site is also accessible by going to the 7000
system home page, selecting "Downloads" and then "Updates
and Patches."
Please note that upgrade CD's will not be distributed.
FURTHER INFORMATION contact Applied Biosystems on:
AUS: 1 800 636 327
NZ: 0 800 446 416
e-mail: abozsupport@appliedbiosystems.com
Please pass this message to any of your colleagues
who may also be interested
***Online Ordering is Now Available***
http://store.appliedbiosystems.com
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SDS
News Bulletin- AB US$250K SNP Grant Announced
SDS Bulletin
February 2003
_____________________________________________
Applied Biosystems ANNOUNCES US$250,000 Grant program
for SNP genotyping studies
Program Will Award up to US$250,000 in Applied Biosystems
Products to an Individual, Institution or Corporation Involved in
SNP Genotyping; Grant Recipient to be Selected by a Panel of Scientific
Experts
FOSTER CITY, CA February 13, 2003 Applied Biosystems
Group (NYSE:ABI), an Applera Corporation business, today announced
a new grant program open to individuals, institutions and corporations
conducting genotyping research using single nucleotide polymorphism's
(SNPs). The grant recipient will receive up to $250,000 in Applied
Biosystems products for SNP genotyping.
The winning proposal for the Applied Biosystems
SNP Genotyping Research Grant will be selected by a panel of scientific
experts, including scientists from Applied Biosystems, as well as
external judges to be announced at a later date. Interested parties
can submit a one- to two-page proposal outlining the goals of their
SNP genotyping project and specific research needs. Application
forms are available at http://www.allsnps.com.
Deadline for submission is April 20, 2003. The winning grant recipient
will be announced in mid-May 2003.
"We hope this grant program will encourage
innovative research in the exciting, growing field of SNP genotyping,"
said Michael W. Hunkapiller, Ph.D., President of Applied Biosystems.
"As a leading provider of tools and services for life science
research, and with the availability of our recent offerings for
SNP genotyping, we look forward to stimulating further advancements
in this field."
The Applied Biosystems Grant Program will provide
the grant recipient with a range of products to choose from within
the Sequence Detection System product line for SNP genotyping, including
Assays-on-Demand(TM) products ready-to-use, functionally validated
assays for nearly 130,000 human SNPs; and instruments for SNP-based
genotyping, including Sequence Detection Systems that enable detection
of single nucleotide polymorphism's (SNPs) using the fluorogenic
5' nuclease assay with TaqMan® chemistry.
About Applera Corporation and Applied Biosystems
Applera Corporation comprises two operating groups.
The Applied Biosystems Group develops and markets instrument-based
systems, reagents, software, and contract services to the life science
industry and research community. Customers use these tools to analyze
nucleic acids (DNA and RNA), small molecules, and proteins to make
scientific discoveries, develop new pharmaceuticals, and conduct
standardized testing. Applied Biosystems is headquartered in Foster
City, CA, and reported sales of $1.6 billion during fiscal 2002.
The Celera Genomics Group (NYSE:CRA), located in Rockville, MD,
and South San Francisco, CA, is engaged principally in integrating
advanced technologies to discover and develop new therapeutics.
Celera intends to leverage its proteomic, bioinformatic, and genomic
capabilities to identify and validate drug targets, and to discover
and develop new therapeutics. Its Celera Discovery System online
platform, marketed exclusively through the Applied Biosystems Knowledge
Business, is an integrated source of information based on the human
genome and other biological and medical sources. Celera Diagnostics,
a 50/50 joint venture between Applied Biosystems and Celera Genomics,
is focused on discovery, development, and commercialization of novel
diagnostic products. Information about Applera Corporation, including
reports and other information filed by the company with the Securities
and Exchange Commission, is available at http://www.applera.com,
or by telephoning 800.762.6923. Information about Applied Biosystems
is available at http://www.appliedbiosystems.com.
Copyright© 2003. Applera Corporation. All rights
reserved. Applied Biosystems is a registered trademark and Applera,
Assays-on-Demand, Celera, Celera Diagnostics, Celera Discovery System,
and Celera Genomics are trademarks of Applera Corporation or its
subsidiaries in the U.S. and certain other countries.
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SDS
Bulletin: cDNA Archive Kit
*** SDS Bulletin
*** 20 June 2001
__________________________________________________________
PRODUCT ANNOUNCEMENT
Hello everyone,
We are pleased to announce the availability of the
new High Capacity cDNA Archive Kit
cDNA is widely used in molecular biology. Many applications
require access to large quantities of cDNA (such as large-scale
gene expression studies, array applications etc.).
The new High Capacity cDNA Archive Kit facilitates
these studies by enabling cDNA conversion from a large input amount
of total RNA (up to 10 ug per tube).
The Kit uses random priming to initiate cDNA synthesis.
This ensures first-strand synthesis occurs efficiently for all RNA
species present. The kit has been validated extensively
for a wide variety of templates including G/C- and
A/U-rich RNA species. We have also examined the effect of mRNA abundance
using samples with both rarely and abundantly
expressed targets.
The RT buffer used is formulated to ensure cDNA
conversion is optimal even for large input amounts of template.
A printed product bulletin is available showing
extensive testing using the 7700 Sequence Detection System (please
request publication 117PB05-01)
___________________________________________________
P/N 4322171 High Capacity cDNA Archive Kit
Sufficient for 200 Reactions up to for up to 10ug
of total RNA each, the kit contains:
* 10 x RT Buffer
* 10 x Random Primers
* MultiScribe reverse Transcriptase
* 25 x dNTPs
P/N 4322169 High Capacity cDNA Archive Kit Protocol
SDS Bulletin-IMPORTANT-TaqMan Probes/Primers Now
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***
SDS Bulletin
*** 7 April 2001
________________________________________________________________
IMPORTANT PRODUCT CHANGE
Effective immediately, the following products will
be prepared dry and shipped ambiently:
- SDS primers
- Prism primers
- Prism primer pairs
- TAMRA Taqman probes
________________________________________________________________
Applied Biosystems has been investigating ways to
speed the synthesis and delivery of custom oligonucleotides from
our OligoFactory in Foster City, CA to our customers in the Australia
and New Zealand region.
We have identified several steps in the ordering-delivery
chain that, when combined, will result in a significant time saving.
The first step in this process is to prepare oligos
in a dry format and ship them ambiently. Stability studies have
been done on several oligo products with no ill effect at being
stored at room temperature. As a result, we can now ship the following
products to you in a dry format. This change was made effective
on April 1, 2001. The product categories are:
- SDS primers
- Prism primers
- Prism primer pairs
- TAMRA Taqman probes
MGB TaqMan probes and linkage marker products will
continue to ship on ice because MGB probes are too difficult to
re-dissolve once dried and the linkage marker products are pre-prepared
and packaged specifically for wet shipment.
This change in procedure will result in a time saving
of several days to 1 week compared to previous delivery timelines.
Future changes such as direct shipment from the
USA to you (drop-ship) and direct web-based ordering (e-commerce)
will result in further significant improvements in delivery times.
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